PURPOSE: Lactobacillus sp. novel microorganism is provided to ensure acid resistance and bile acid resistance, to replace existing antibiotics as probiotics which suppress pathogenic bacteria, and to treat or prevent symptoms caused by abnormal fermentation of harmful microorganisms in intestine when administering to livestock. CONSTITUTION: A probiotic composition containing one or more kinds of strains or a culture thereof is selected from the group consisting of Lactobacillus salivarius KACC91678P, Lactobacillus plantarum KACC91679P, Lactobacillus plantarum KACC91680P, and Lactobacillus amylovorus KACC91681P. An additive for animal feed contains the composition. A method for preventing or treating diarrhea by microorganism or suppressing growth of pathogenic microorganisms in intestine comprises a step of administering the composition to animals exclusive of human.본 발명은 장내 유해한 병원균을 억제하고, 장 세포 부착이 우수하고, 면역증강 효과가 있으며, 내성 전이가 없고, 내산성 및 내담즙성이 우수한 신규 락토바실러스 속 유산균 및 이의 생균제로서의 용도에 관한 것이다. 상기 생균제는 가축의 사료 첨가제 및 수의학적 조성물로 유용하게 사용될 수 있다.