Compositions and methods for disinfesting rooms and objects through a mist are disclosed. The compositions generally include a bactericidic and/or parasiticidic substance, a poly-acid or hydrophilic sulfone, and optionally a cell membrane polarizing component. By using an organic poly-acid or hydrophilic sulfone in a composition containing the bactericidic and/or parasiticidic substance, such as hydrogen peroxide or akacid, for disinfecting rooms and objects through a mist of said composition, it is possible also to kill mycobacteria.Selon l'invention, en utilisant du diméthylsulfone dans une composition contenant du peroxyde d'hydrogène pour la désinfection de pièces et d'objets par l'intermédiaire d'un brouillard de ladite composition, il est également possible de détruire les mycobactéries.