FREMSTILLINGSFREMGANGSMÅDER TIL AT STYRE C-TERMINAL LYSIN, GALACTOSE OG SIALINSYREINDHOLD I REKOMBINANTE PROTEINER

• 收藏
• 分享

专利权人:

Janssen Biotech, Inc;Janssen Biologics B.V.

发明人:

FLIKWEERT, Marcel,GOOCHEE, Charles,MASLANKA, Francis,NAGEL, Francisus Johannes Ignatius,RYLAND, James,SCHAFER, Eugene

申请号：

DK20140768761T

公开号：

DK2970980(T3)

申请日:

2014.03.07

申请国别(地区):

丹麦

年份:

2018

代理人:

摘要：

Provided herein is a method for producing an antibody, such as an anti-TNFα antibody (e.g., infliximab) having a C-terminal lysine content of about 20% to about 70%, and a sialic acid content of about 1% to about 20%, comprising culturing a zinc-responsive host cell transfected with DNA encoding the antibody in a culture medium comprising at least 0.5 μM zinc; and controlling the concentration of zinc in the culture medium, thereby producing the antibody.

来源网站：

中国工程科技知识中心

来源网址：

http://www.ckcest.cn/home/