SLEPNEV, Vladimir, I.,KODVAWALA, Ahmer,LOEFFLER, Brian,HINDUPUR, Anand,PONAKA, Reddy,ELAGIN, Vecheslav, A.
申请号：
DK20130794485T
公开号：
DK2854981(T3)
申请日:
2013.05.24
申请国别(地区):
丹麦
年份:
2018
代理人:
摘要：
The invention provides methods of detecting a nucleic acid present in a biological sample, comprising combining the biological sample with a lysis buffer to form a lysis mixture comprising nucleic acid released from cells in said biological sample; and subjecting a volume of the lysis mixture to size-exclusion chromatography in a column comprising a volume of size-exclusion medium. In certain embodiments, the lysis buffer separates double-stranded nucleic acid into single-stranded nucleic acid. In certain embodiments, the elution can have a flow rate of separation of less than 10 minutes to produce an eluted solution comprising isolated nucleic acid. The invention provides for a method of accurately and rapidly detecting products of nucleic acid amplification.
