An image pickup apparatus includes: an optical unit including an incident surface and an emission surface, in which a plurality of optical members are arranged around an optical axis; a polygonal prism image pickup unit including a light receiving surface, a rear surface and a plurality of side faces, in which a plurality of elements including an image pickup device configured to photoelectrically convert light made incident from the optical unit and output an image pickup signal are laminated; and at least one guide, a front portion of which is fixed to the emission surface of the optical unit and a rear portion of which is provided with a plurality of contact surfaces that are respectively in contact with and fixed to the plurality of side faces of the image pickup unit.