The present invention provides a symmetrical temperature difference acquisition method for performing a long-term human temperature monitoring on multiple locations at the same time, which is applicable to precise medical treatments. The technical means of the present invention is characterized by the following steps: a preparation step for preparing a temperature sensing device including a first sensing set and a second sensing set, each of the first and the second sensing sets having at least one temperature sensing patch a measurement step for placing the temperature sensing device to a predetermined location on a person under measurement in order to acquire temperature variation data from the predetermined location on the person under measurement, wherein the predetermined location includes a first predetermined location group and a second predetermined location group and a data extraction step for removing the temperature sensing device when it operates for a certain period of time to complete acquisition of the temperature variation data and then stops operation due to low power, and connecting the temperature sensing device to a reading device so as to extract temperature variation data from each temperature sensing patch, thereby enabling medical personnel to apply the extracted temperature variation data to the symmetrical precise medical treatment.本發明在於提供一種多點同時進行,對人體溫度長時間監測,能應用於精準醫療上的對稱溫差取得方法。其技術手段:其特徵在於包括以下步驟:準備步驟:準備溫度感測裝置,其包括第一、二感測組,而第一、二感測組皆包括至少一溫度感測貼片;測量步驟:將溫度感測裝置,對應放置定位到被測人員的預定位置處,以收集被測人員之預定位置處的體溫變化數據,預定位置包括第一、二預定位置組群;以及提取步驟:當前述溫度感測裝置於一定時間後,因電力耗盡而停止運作時,即體溫變化數據收集完畢,將溫度感測裝置取下,連接讀取裝置以將儲存於各個溫度感測貼片內的體溫變化數據取出,讓醫療人員能將體溫變化數據,應用於對稱精准醫療上。I‧‧‧準備步驟Ⅱ‧‧‧測量步驟Ⅲ‧‧‧提取步驟