A kind of Temperature and Humidity Control culture systems of energy-saving operation,The culture systems include a temperature control circuit and an incubator,Wherein,One of temperature control circuit compressor both ends are connected with a first heat exchanger and a second heat exchanger respectively,Compression processing is carried out to enable compression function docking receive from the low temperature refrigerant of the first heat exchanger,And the high temperature refrigerant of compression processing Hou is made to flow into the second heat exchanger; The first cooler and warmer system in the incubator penetrate one first cooling circuit respectively and a heating circuit is connected with the first heat exchanger and second heat exchanger respectively; Flow control valve of the temperature and humidity controller system respectively with the first cooling circuit and heating circuit is mutually electrically connected one of in the incubator,With according to the temperature and humidity in the culture space detected,The flow of one of first cooling circuit first fluid is controlled respectively,And the flow of one of heating circuit second fluid. In this way, first cooler and warmer just can be penetrated, regulates and controls the temperature or humidity in the incubator, without being additionally configured with a heater in the culture space, therefore, can effectively reach the environmentally protective purpose of carbon reduction.一種節能運轉之溫濕度控制培養系統,該培養系統包括一控溫迴路及一培養箱,其中,該控溫迴路之一壓縮機兩端分別與一第一熱交換器及一第二熱交換器相連接,以令該壓縮機能對接收自該第一熱交換器之低溫冷媒進行壓縮處理,並使壓縮處理後之高溫冷媒流入該第二熱交換器;該培養箱內之第一冷卻器及加溫器係分別透過一第一冷卻迴路及一加溫迴路分別與該第一熱交換器及第二熱交換器相連接;該培養箱內之一溫濕度控制器係分別與第一冷卻迴路及加溫迴路之流量控制閥相電氣連接,以根據所偵測到之該培養空間內的溫度及濕度,分別控制該第一冷卻迴路中之一第一流體的流量,及該加溫迴路中之一第二流體的流量。如此,便能透過該第一冷卻器及加溫器,調控該培養箱內的溫度或濕度,而無需在該培養空間內另外裝設一加熱器,因此,能有效達成節能減碳的綠色環保目的。