Hyeon-Je Cho,Linda A. Castle,Theodore M. Klein,Tai Minh,York Moy
申请号:
US13438077
公开号:
US20120255070A1
申请日:
2012.04.03
申请国别(地区):
US
年份:
2012
代理人:
摘要:
Compositions and methods are provided for the efficient transformation of a dicot plant. More particularly, compositions and methods of the present invention find use in agriculture for Agrobacterium-mediated transformation of a dicotyledonous plant. The compositions include cultivation media comprising high concentrations of sucrose and glucose. The cultivation media find use in methods directed to Agrobacterium-mediated transformation of a dicot plant with a gene of interest. In this manner, any gene of interest can be introduced into a dicot plant with high transformation efficiency and reduced tissue necrosis.