PURPOSE: A method for preparing a conjugate and nanoparticles is provided to ensure uniform particle distribution and high stability and to enhance solubility.CONSTITUTION: A conjugate for preparing drug delivery nanoparticles contains hydrophilic gelatin, and hydrophobic oleic acid conjugated with gelatin through amide bond. The conjugate further contains folic acid conjugated to the gelatin. A method for preparing gelatin and conjugate containing the gelatin and oleic acid comprises: a step of dissolving the gelatin in water to prepare a solution containing gelatin and a step of adding colloidal water dispersion solution containing oleic acid and monoethanol amine to the solution containing gelatin. A method for preparing gelatin and a conjugate containing oleic acid and folic acid comprises: a step of dissolving the gelatin in the water to prepare a solution containing gelatin a step of adding the colloidal water dispersion solution to the solution containing gelatin to prepare a solution containing the gelatin and a first conjugate with oleic acid conjugated with the gelatin a step of drying and preparing the first conjugate from the solution a step of adding a buffer solution to the first conjugate and a step of imide compounds and folic acid to the buffer solution.COPYRIGHT KIPO 2013[Reference numerals] (AA,EE,GG) Gelatin (BB,FF,HH) Oleic acid (CC,DD,II) Folic acid본 발명은 젤라틴 및 젤라틴에 결합한 올레산을 포함하는 접합체, 상기 접합체를 포함하는 나노입자, 약물이 봉입된 나노입자 및 접합체와 나노입자를 제조하는 방법을 제공한다. 본 발명에 따른 접합체를 사용하여 제조된 나노입자는 약물봉입율이 우수하고 균일한 입자 분포도 및 높은 안정성을 나타내며 난용성의 약물을 높은 용해도로 수용액에 용해시킬 수 있다.
