The present invention provided an anticancer nano-silver composition for the treatment of lung cancer. The composition contains spherical nano-silver powder 12-200 mg/kg, pharmaceutically acceptable dispersing agent carbomer 700-800 mg/kg, triethanolamine 700-800 mg/kg, glucose 2.8-3.2 g/kg, and purified water as the diluent of the spherical nano-silver powder, the purity of silver is ≧99.99% and the particle size is 1-5 nm. Experiments demonstrated that the anticancer nano-silver composition can be used to inhibit proliferation of the human non-small cell lung cancer A549, and cause all cells death. The nano-silver composition of the present invention can be used in the manufacture of medicaments and for the treatment of lung cancer.