PROBLEM TO BE SOLVED: To provide a germination treatment method of Viola thibaudieri, which promises a contribution to enhancement of the individual propagation or survival rate of Viola thibaudieri. ! SOLUTION: In a germination treatment method of Viola thibaudieri, seeds collected from Viola thibaudieri are held in a moisture retaining atmosphere substantially without nutrition, and a multistage germination treatment as one cycle is repeated in one ore more cycles until the seeds are rooted and germinated, and the roots are extended to a predetermined length. The multistage germination treatment is composed of a three-step temperature treatment consisting of (1) a high-temperature treatment for holding the seeds at a predetermined temperature in a high-temperature range of 15-25掳C for 3-1 months, (2) a middle-temperature treatment for holding them at a predetermined temperature in a middle-temperature range of 5-15掳C for 2.5-1.5 months, and (3) a low-temperature treatment for holding them at a predetermined temperature in a low-temperature range of 1-5掳C for 4-2 months; and a temperature raising treatment. ! COPYRIGHT: (C)2013,JPO&INPIT
