< Topic >The method where it differentiates induces skeletal muscle or the skeletal muscle fore-quarter cell is offered from the versatile characteristic trunk cell.SolutionsProcess below, (1) the process which it floats cultures the human versatile characteristic trunk cell, (2) the process which it glues cultures the cell population after the floating culturing, (3) process, and (4) the cell after the gluing culturing is dissociated the process which it glues cultures the cell which was made to dissociate is included, artificial skeletal muscle or the induction skeletal muscle fore-quarter cell which is produced by method, and this method artificial skeletal muscle or the skeletal muscle fore-quarter cell being produced from human versatile characteristic trunk cell is offered.< Selective figure >Figure 1【課題】多能性幹細胞から骨格筋または骨格筋前駆細胞を分化誘導する方法を提供する。【解決手段】以下の工程、(1)ヒト多能性幹細胞を浮遊培養する工程、(2)浮遊培養後の細胞集団を接着培養する工程、(3)接着培養後の細胞を解離する工程、および(4)解離させた細胞を接着培養する工程を含む、ヒト多能性幹細胞から人工の骨格筋または骨格筋前駆細胞を製造する方法、ならびに、この方法によって作製された人工の骨格筋または誘導骨格筋前駆細胞を提供する。【選択図】図1
