The present invention provides an integrated fruits and vegetables and berries ferment, and preparation method thereof and use thereof. The three-stage extraction process of the present invention can improve the total polyphenol content and/or the activity of superoxide dismutase (SOD) of the integrated fruits and vegetables and berries juice, and therefore the integrated fruits and vegetables and berries ferment can be used for preparing a composition for enhancing antioxidant activity； wherein the three-stage extraction process comprises a first aqueous phase extraction step, a second alcohol phase extraction step, and a third organic acid phase extraction step.本發明提供一種蔬莓果發酵物的製備方法及其用途，本發明之三段式萃取製程能提升蔬果汁及莓果汁之混合物的總多酚的含量、及/或超氧化物歧化酶的活性，而可將以此製程所得之本發明蔬莓果發酵物用於製備提升抗氧化活性之組合物的用途；其中，該三段式萃取包含第一段水相萃取步驟、第二段醇相萃取步驟、及第三段有機酸相萃取步驟。
