用於藥物傳遞之脂質奈米粒子的製造方法农业专利-农业学术服务平台

发明人:: KNOPOV, VICTOR,诺波夫　维克多,諾波夫　維克多,WITTE, RICHARD P.,威特　理查Ｐ,KARMALI, PRIYA,卡马利　佩利亚,卡馬利　佩利亞,LEE, ROBIN,李　罗宾,李　羅賓,WEBB, DAVID,韦伯　大卫,韋伯　大衛,AKOPIAN, VIOLETTA,寇派恩　凡欧里特,寇派恩　凡歐里特,诺波夫维克多,諾波夫維克多,威特理查P,卡马利佩利亚,卡馬利佩利亞,李罗宾,李羅賓,韦伯大卫,韋伯大衛,寇派恩凡欧里特,寇派恩凡歐里特

摘要：: What is described is a method for preparing a liposome that efficiently encapsulates a negatively charged therapeutic polymer, e.g., siRNA. The process involves preparing a lipid mixture comprising a cationic lipid in a water miscible organic solvent such as ethanol, and injecting this solution to the polymer dissolved in water to a final concentration of 35% ethanol in water to produce nanoparticles having a mean size of 50 to 150 nm and a final charge ratio of drug:lipid is 1:2.5.本發明揭示一種用於製造可有效囊封負電荷治療性聚合物(例如siRNA)之脂質體的方法。該方法涉及以可與水混溶之有機溶劑(諸如乙醇)製備含陽離子脂質之脂質混合物，及將該溶液注入至已溶於水中之聚合物中，以使溶於水中達35%乙醇之最終濃度，從而製得具有50至150 nm平均粒徑及藥物：脂質最終電荷比為1：2.5之奈米粒子。