PROBLEM TO BE SOLVED: To provide a royal jelly-decomposing enzyme most suitable for obtaining decomposed products having a high physiological activities such as a functional peptide, etc., from the royal jelly in a good efficiency, and to provide a material containing a royal jelly-decomposing enzyme.SOLUTION: This material containing royal jelly-decomposing enzyme is prepared by a method including the following processes from the 2-3 day old queen bee larvae of Apis mellifera: (a) a process of centrifugal treatment of the suspension of body tissues of the queen bee larvae at &le6°C so as to separate to an upper layer of white solid form, a middle layer of solution and a lower layer of precipitate and (b) a process of recovering the middle layer as the material containing royal jelly-decomposing enzyme. The royal jerry-decomposing enzyme is characterized by being contained in the above material containing royal jerry-decomposing enzyme. The royal jelly-decomposed products are prepared by using the material containing royal jelly-decomposing enzyme or the royal jelly-decomposing enzyme. The method for decomposing the royal jelly is characterized by using the material containing royal jelly-decomposing enzyme or the royal jelly-decomposing enzyme.COPYRIGHT: (C)2009,JPO&INPIT【課題】ローヤルゼリーから、機能性ペプチド等の生理活性の高い分解物を効率よく得るために最適なローヤルゼリー分解酵素、及び、該ローヤルゼリー分解酵素含有物の提供。【解決手段】西洋ミツバチ (Apis mellifera) の2~3日齢の女王蜂幼虫から、下記工程を有する方法で調製されたローヤルゼリー分解酵素含有物;(a)前記女王蜂幼虫の体組織懸濁物を6℃以下で遠心処理することにより、白色固形の上層、溶液の中層、沈殿による下層の3層に分離する工程(b)前記中層をローヤルゼリー分解酵素含有物として回収する工程、該ローヤルゼリー分解酵素含有物に含有されることを特徴とするローヤルゼリー分解酵素、該ローヤルゼリー分解酵素含有物又は該ローヤルゼリー分解酵素を用いて調製されたローヤルゼリー分解物、及び、該ローヤルゼリー分解酵素含有物又は該ローヤルゼリー分解酵素を用いることを特徴とするローヤルゼリーの分解方法。【選択図】なし