Methods for the production of a cosmetic composition from hatching fluid of salmonidae eggs and uses thereof for improving the cosmetic appearance of skin
Use of a cosmetic composition in the manufacture of a medicament for reducing or preventing the cosmetic appearance or prevalence of wrinkles, fine lines, hyperpigmentation, laxity, dry skin, scaling and/or transepidermal water loss in skin of a mammalian animal, wherein the composition is obtained by a method comprising at least the steps of: a) suspending Salmonidae eggs in a minimal volume of water； b) inducing synchronized, rapid hatching of the eggs； c) filtering the hatched eggs to obtain hatching fluid； and d) filtering the hatching fluid to obtain the composition, wherein the step of filtering the hatching fluid comprises at least the steps of: (i) filtering the hatching fluid using a filter with a pore size of at least 5&mu；m, and collecting the filtrate； (ii) filtering the filtrate from step (i) using a filter with a pore size of 0.30-0.60&mu；m, and collecting the filtrate； (iii) exchanging the water in the filtrate from step (ii) with a pharmaceutically acceptable buffer by performing diafiltration using a filter with an exclusion size of less than 15kDa； (iv) optionally repeating the diafiltration step of step (iii)； (v) optionally diluting the solution from step (iii) or (iv) such that the solution has an enzymatic activity of 3000-5000 mU/L, measured by the capacity of the solution to cleave the Factor Xa chromogenic substrate (CH3OCO-D-CHA-Gly-Arg-pNA-AcOH)； (vi) filtering the solution from step (iii), (iv) or (v) using a filter with a pore size of 0.15-0.30&mu；m, and collecting the filtrate； and (vii) preparing the cosmetic composition from the filtrate from step (vi).
