A containment device for biological samples, which comprises:- a first vessel (11) for depositing a biological sample,- a lid (12) adapted to engage the first vessel (11),- a hermetically-sealed chamber (13), adapted to contain a preservative, arranged between the lid (12) and the first vessel (11),- means of generating a passage between the hermetically-sealed chamber (13) and the first vessel (11) for the descent of the preservative from the chamber to the first vesselthe hermetically-sealed chamber (13) is defined by a second vessel (14), which is inserted into the first vessel (11) with the filling opening (15) directed toward the lid (12), and by the lid (12) itself, closed onto the first vessel (11),- the means of generating a passage between the hermetically-sealed chamber (13) and the first vessel (11) comprise a region (18) with a pre-fracture (19) that is defined on the second vessel (14) and a corresponding presser (20) that extends from the lid (12) and is in a position to encounter the region (18) with a pre-fracture (19)- the first vessel (11) and the lid (12) are mutually engaged with coupling means that define either a first configuration of use, in which the lid (12) is closed and the second vessel (14) is intact, or a second configuration of use, in which the lid (12) is closed and is displaced toward the inside of the first vessel (11) and the presser (20) is arranged so as to interrupt the continuity of the second vessel (14) at the region (18) with a pre-fracture (19).
