The present invention provides a nano emulsion injection containing vinca alkaloids, in particular containing vinorelbine and its preparation method. The mean diameter of present nano emulsion is less than 100nm which bearing the characteristics of reducing injection irritation of vinorelbine, increasing vinorelbine concentration in the preparation and higher targeting function.本發明提供一種含有長春花生物鹼,特別是含有長春瑞濱的奈米乳劑注射液及其製備方法。本發明奈米乳劑注射液的平均粒徑小於100nm,既能減小長春瑞濱注射液的用藥刺激性,又可以提高長春瑞濱在產品中的濃度,同時具有更高的靶向性。無元件符號