The present invention provides a pot seedling feeding system for a transplanter, including a conveyor unit, a detection sensor, a controller, a seedling separation unit, side plates, and support stands. The conveyor unit includes a step motor, a transmission shaft, a conveyor belt, a tensioning shaft, and bearings. The seedling separation unit includes a seedling separation rod, a reset spring, a guide post, and a support seat. The controller is used to control the step motor which drives the conveyor belt in order to move the pot seedling. When a seedling receiving barrel of the planter rises to a seedling receiving position, the planter is controlled by using a brake cable, the seedling separation rod is then ejected which in turn drives the step motor to carry a pot seedling to move forward. The pot seedling is then dropped in a seedling receiving cup of the planter. In order to improve the quality of pot seedling planting, the seedling separation rod corrects a feeding posture of the pot seedling at the m