The reducing surgical stress and operative time eliminates a major biological surgery, to provide a biological tube ostium suturing surgical system having excellent operability of the operation, safety and reliability. The present invention, the suture member sutures 100-120 103-143 is associated freely pulled out at the rear end of the arrow members 101-121 of the pair is locked to the biological tissue tubes in the tube member 51 of the pair of parallel and suture member 5~5B cartridge that houses each, and shooting auxiliary section 3~3B with a plunger at both ends opening cylinder 31,31B within a pair, biological insertion tube body 2 made of air line 21 of the pair of flexible, Gasushuta 4 are implanted on both sides within the organization of small holes Tc part of a living body tube T in by injection sequentially Arrow member by the. 2A, is injected sequentially at both ends opening cylinder high-pressure gas or compressed air by 44~4B4 operation trigger and ~4B, There are respectively connected in sequence, a biological tube ostium suturing surgical devices 1,1A for suturing to close contact with the small hole portion tissues of a living body tube using a combination of endoscopic 200,200A that is inserted into a biological tube provided. [Selection Figure Figure 2大掛かりな生体手術を省き手術時間と手術侵襲を低減し、手術の操作性、安全性、信頼性に優れる生体管小孔縫合手術システムを提供する。本発明は、並列する一対の管部材51内に生体管組織に係止される一対のアロー部材101~121の後端に縫合糸103~143が引出し自在に連係された縫合部材100~120をそれぞれ収容する縫合部材カートリッジ5~5Bと、一対の両端開口シリンダ31、31B内にそれぞれプランジャを備えるシューティング補助部3~3Bと、可撓性の一対の送気管21からなる生体管内挿入本体2、2Aと、トリガー44~4B4操作により圧縮エア又は高圧ガスを両端開口シリンダ内に順次噴射することによりアロー部材を順次射出して生体管T内の小孔部Tc部位の両側組織内に打ち込むガスシュータ4~4Bと、が順次それぞれ連結され、生体管内に挿入された内視鏡200、200Aを併用して生体管の小孔部部位組織を密着させ縫合する生体管小孔縫合手術装置1、1Aを具備する。【選択図】 図2