UNIVERSIDAD CATOLICA DE CORDOBA;CONSEJO NACIONAL DE INVESTIGACIONES CIENTIFICAS YTECNICAS (CONICET)
发明人:
LUJAN HUGO DANIEL
申请号：
NZ60494309
公开号：
NZ604943A
申请日:
2009.12.02
申请国别(地区):
NZ
年份:
2014
代理人:
摘要：
Disclosed is a vaccine composition for use against infections produced by Giardia lamblia, wherein the composition comprises: a modified Giardia lamblia parasite in which a gene encoding Dicer, a gene encoding RNA&ndash；dependant RNA&ndash；polymerase (RdRP), or both have been silenced, wherein said modified Giardia lamblia comprises simultaneous expression on its surface of more than one variable surface protein (VSP), and pharmaceutically acceptable excipients and/or adjuvants. Further disclosed is vaccine composition, comprising: the complete repertoire of variable surface proteins (VSP) of a modified Giardia lamblia parasite in which a gene encoding Dicer, a gene encoding RNA&ndash；dependant RNA&ndash；polymerase (RdRP), or both have been silenced, wherein said proteins are obtained employing the procedure comprising the following steps: a) linking an antibody recognising the VSP CRKGA amino acid sequence to a solid support； b) contacting the solid support with said modified Giardia lamblia； and c) isolating the complete repertoire of variable surface proteins (VSP)； and pharmaceutically acceptable excipients and/or adjuvants. Further disclosed is the use of a modified Giardia lamblia parasite in preparing a vaccine composition, wherein a gene encoding Dicer, a gene encoding RNA&ndash；dependant RNA&ndash；polymerase (RdRP), or both have been silenced in said parasite, and wherein said modified Giardia lamblia comprises simultaneous expression on its surface of more than one variable surface protein (VSP). Further disclosed is the use of at least two variable surface proteins isolated from a modified Giardia lamblia parasite in preparing a vaccine composition, wherein a gene encoding Dicer, a gene encoding RNA&ndash；dependant RNA&ndash；polymerase (RdRP), or both have been silenced in said parasite, and wherein said modified Giardia lamblia comprises simultaneous expression on its surface of more than one variable surface protein (VSP).
