The purpose of the present invention is to provide a method for producing ³´T cells with which it is possible to obtain a cell population including a large amount of y´T cells and including virtually no cells infected by human T-cell leukemia virus (HTLV) even when PBMCs derived from a person infected with HTLV are used as the cell source. A cell population including a large amount of y´T cells and including virtually no HTLV-1-infected cells is obtained by culturing after removing CD4-positive cells that express CD4 (referred to hereinafter as "CD4 + cells") and CD8-positive cells that express CD8 (referred to hereinafter as "CD8 + cells") from the PBMCs that are used as the cell source, or after removing cells that express ±²T cell receptors (±²TCR) (referred to hereinafter as "±²T cells"), before expanding ³´T cells.
