The United States of America; as represented by the Secretary; Department of Health and Human Serv
发明人:
Udai S. Kammula
申请号：
US14268147
公开号：
US20140234353A1
申请日:
2014.05.02
申请国别(地区):
US
年份:
2014
代理人:
摘要：
The invention provides a method of obtaining a population of antigen-specific T cells from peripheral blood of a host. An embodiment of the method of the invention comprises (i) dividing PBMCs from peripheral blood of a host into more than one sub-population； (ii) contacting the PBMCs with an antigen and IL-2； (iii) obtaining a sample of PBMCs from each sub-population； (iv) identifying an antigen-reactive sub-population by determining by high throughput quantitative PCR the expression of a factor produced by the PBMCs of each sample； (v) dividing the antigen-reactive sub-population into microcultures； (vi) identifying the antigen-reactive microculture； and (vii) expanding the microculture, thereby obtaining a population of T cells specific for the antigen. The invention also provides a population of T cells obtained by the inventive method, a pharmaceutical composition comprising the same, and a method of treating a disease in a host using the pharmaceutical composition. Related isolating and screening methods are further provided.
