A non-human animal is genetically modified with sequence encoding a human or humanized MHC molecule or associated molecule, e.g., β2 microglobulin, and expression of the sequence by the non-human animal induces tolerance to the corresponding human HLA from which the human or human MHC molecule is derived.The tolerance exhibited by these non-human animals allows these animals to generate specific antibody responses to the corresponding human HLA when such HLA is presenting a peptide that is antigenic to the non-human animal.Such an antibody response, which specifically targets a pMHC complex of interest without binding to the MHC molecule may be useful in immunotherapeutic modalities that target a component of the immunological synapse which provides the specificity of that interaction.一種非人類動物經基因修飾具有編碼人類或人類化MHC分子或相關分子(例如β2微球蛋白)之序列，且由該非人類動物表現該序列誘導對衍生該人類或人類MHC分子之對應人類HLA的耐受性。當對應人類HLA呈現針對該非人類動物具有抗原性的肽時，由此等非人類動物展現之該耐受性允許此等動物產生針對此類HLA之特異性抗體反應。特異性地靶向所關注之pMHC複合物而不結合於該MHC分子之此抗體反應可適用於靶向提供該相互作用之特異性的免疫突觸之組分的免疫治療模式中。
