Provided are a diagnosis assisting device that facilitates a user to grasp adifferenceof an affected area to perform a highly precise diagnosis assistance, an imageprocessingmethod in the diagnosis assisting device, and a program. An image processingmethod in adiagnosis assisting device that diagnoses lesions from a picked-up imagecomprisesperforming an image correction on the picked-up image for diagnosis, andsubtracting atarget pixel value from a pixel value of the picked-up image having undergonethe imagecorrection to obtain an input image to an identifier that identifies diseasesbased on theunknown picked-up image relating to an object to be diagnosed.