PROBLEM TO BE SOLVED: To provide a production method of γδT cells by which a cell population hardly comprising HTLV infected cells and comprising a large amount of γδT cells even if using PBMCs derived from HTLV (human T cell leukemia virus) infected persons as cell sources can be obtained.SOLUTION: The cell population which hardly comprises HTLV-1 infected cells and which comprises a large amount of γδT cells can be obtained by culturing after removing CD4 positive cells which express CD4 (hereinafter they are described as "CD4+ cells"), and CD8 positive cells which express CD8 (hereafter they are described as "CD8+ cells"), or after removing cells which express αβ type T cell receptor (αβTCR) (hereafter they are described as "αβT cells"), from PBMCs which are the cell sources, before proliferating γδT cells.SELECTED DRAWING: NoneCOPYRIGHT: (C)2016,JPO&INPIT【課題】HTLV(ヒトT細胞白血病ウィルス)感染者由来のPBMCsを細胞源として用いた場合であっても、HTLV感染細胞をほとんど含まず、かつγδT細胞を大量に含む細胞集団を得ることができる、γδT細胞の製造方法を提供することを目的とする。【解決手段】γδT細胞を増殖させる前に、細胞源となるPBMCsからCD4を発現するCD4陽性細胞(以下、「CD4+細胞」と記す)とCD8を発現するCD8陽性細胞(以下、「CD8+細胞」と記す)を除去した後、又はαβ型T細胞受容体(αβTCR)を発現する細胞(以下、「αβT細胞」と記す)を除去した後に培養を行うことで、HTLV-1感染細胞をほとんど含まず、かつγδT細胞を大量に含む細胞集団を得られる。【選択図】なし
