A vaginal cleaner and manufacture method thereof. The manufacture method includes following steps: inoculating a Lactobacillus strain into a broth for forming a Lactobacillus culture. Then, incubating the Lactobacillus culture at an 30-40℃ environment and adding a fermentation accelerator to react with the Lactobacillus strain. Next, centrifuging the Lactobacillus culture for separating a fermentation broth and a pellet. After that, collecting the fermentation broth having a hydrogen peroxide and a bacteriocin, and finish a manufacture of the vaginal cleaner.一種女性私密處清潔劑及其製造方法,此方法係先將乳酸菌菌株接種於培養液,使形成乳酸菌培養液。接著於30℃至40℃的環境中培養此乳酸菌培養液,並加入發酵促進劑與乳酸菌菌株行生化反應,縮短發酵所需時間。然後,將培養後之乳酸菌培養液執行離心操作,以得到乳酸菌發酵液及菌體。接著,收集乳酸菌發酵液,此乳酸菌發酵液具有一過氧化氫及一抑菌素,而完成女性私密處清潔劑的製備。