ABSTRACT The present invention relates to a pharmaceutical composition containing platelet product and a process of preparation thereof. The pharmaceutical composition containing platelet lysate is in lyophilized form with an improved growth factor from activated platelets and with quantification of the important growth factors needed for healing. It further comprises additives and platelet reagent in which the platelet lysate as an active agent and the platelet lysate are broken to release growth factors. Further, the present invention relates to the pharmaceutical composition with improved sterility by pathogenic DNA removal/damage, thereby enhancing patient safety. Further, the present invention relates to a process of preparation of the pharmaceutical composition.