JUNO THERAPEUTICS, INC.;CHURCH, Sara Elizabeth;GUNTHER, Jon Charles;POLLOCK, Kathryn
发明人:
CHURCH, Sara Elizabeth,GUNTHER, Jon Charles,POLLOCK, Kathryn
申请号：
WO2018US22522
公开号：
WO2018170188(A3)
申请日:
2018.03.14
申请国别(地区):
世界知识产权组织国际局
年份:
2018
代理人:
摘要：
The present disclosure relates to methods comprising cryogenically storing cells from a biological sample derived from a donor, in particular an apheresis sample, wherein the cells are frozen in a controlled rate freezer using a stepwise freezing profile comprising at least one step wherein the sample and/or chamber is cooled at a rate greater than 1° C per minute, and wherein the cells may comprise or be enriched for T cells. Further aspects relate e.g. to the point in time at which the cells are obtained from the donor, the storage time, shipping the cells to a storage facility before or after cryogenic freezing, the sample container being marked with codes or identifiers for cataloging the cells, administering a therapeutically effective amount of a composition comprising engineered T cells generated from the cryogenically frozen cells to a subject in need thereof; and treatment of the donor for a disease, in particular cancer.
