PURPOSE: A germination method of soybean seeds is provided to manufacture germinated soybeans having the increased content of GABA and a high concentration of calcium and iron. CONSTITUTION: Chitosan with a low molecular weight is dissolved in a glutamic acid solution. The chitosan solution is used as supply water in germination of soybeans. The soybeans are dipped in the distilled water at 25 deg. C for 4 hours. The dipped soybeans are strained and germinated at 25 deg. C for 24-72 hours. The distilled water is replaced with a calcium dissolved solution so that calcium is absorbed in the root of the germinated soybeans. The distilled water is replaced with an iron dissolved solution so that iron is absorbed in the root of the germinated soybeans at pH 6. Calcium is CaCl2. The concentration of CaCl2 is 1,500mg/L. [Reference numerals] (AA) Germination percent(%)； (BB) Germination time； (CC) Germination length(cm)； (DD) Chitosan molecular weight본 발명은 GABA(γ-aminobutyric acid) 함량이 증대된 대두종자 발아법에 관한 것으로 대두를 4시간 수침한 후 25℃에서 3일간 발아시키므로서 24시간 증대된 항산화 활성수준이 유지되며, 키토산과 glutamic acid를 처리하는 경우 발아대두의 GABA함량은 2,903μmol/g로 일반대두의 2배에 달하는 것으로 나타났고 발아콩의 칼슘 함량을 증대시키기 위하여 콩의 발아 후 1500mg/L의 염화칼슘 용액으로 발아수를 교체한 결과 칼슘이 17% 증가하며, 철분의 경우 pH6 용액에서 철분을 용해하여 발아하였을 때 철분함량이 35% 증가하였다.
