matriz reticulada, método para controlar a formação de uma matriz, método ou matriz, método para vedar um tecido contra vazamento de um fluído corporal, agente hemostático ou vedante cirúrgico, composição para vedar um ferimento, uso da composição, composição para um veículo para entrega localizada de fármaco, composição para engenharia de tecido, e método para modificar uma composição
A modified enzyme molecule, comprising a modified transglutaminase enzyme molecule with a modification that increases a perceived volume of said modified enzyme molecule, wherein said modification is selected from the group consisting of a modification that increases an actual size of said modified enzyme molecule a modification that increases a hydrodynamic volume of said modified enzyme molecule and a modification that modifies an electrostatic charge of said modified enzyme molecule to be of opposite sign to a net charge of said substrate polymer by changing the isoelectric point (pi) of said modified enzyme in comparison to unmodified enzyme wherein said increase in said perceived volume prevents excessive cross-linking of a substrate by said modified enzyme molecule, wherein said excessive cross-linking leads to a reduction in mechanical properties of cross-linked substrate, or results in reduced elution of said modified enzyme molecule from said cross-linked substrate.
