An image analysis apparatus includes: a calculator that generates, on the basis of a plurality of sample images obtained by irradiating a biological tissue with light having N wavelength bands selected from a wavelength bandwidth from 950 nm or more to 1600 nm or less as a predetermined wavelength bandwidth, sample data on the tissue in the predetermined wavelength bandwidth and a data generator that compares the sample data with training data on a substance that is a training target, to generate distribution data indicating a distribution of the substance in the tissue.
