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Agents for the control of Limnoperna sp.
专利权人:
MARRONE BIO INNOVATIONS; INC.
发明人:
RACKL SARAHANN,MARRONE PAMELA,ASOLKAR RATNAKAR,KOIVUNEN MARJA,HUANG HUAZHANG
申请号:
ARP110104140
公开号:
AR083811A1
申请日:
2011.11.04
申请国别(地区):
AR
年份:
2013
代理人:
摘要:
Requirement 1: a method of controlling a restricted species, including the import of at least one of the following products (a) cell suspensions containing cells that produce the toxin properties of the species at the place where control is sought Pseudodominia (b) one or more substances that (a) are toxic to limnoperma species, and (b) are derived from pseudodom species or cell suspensions, resulting from effective pseudodom species that control these limnoperma species.The substance is selected from a group of substances, including: (a) a compound with a molecular weight of about 1280-1310 determined by liquid chromatography / mass spectrometry (LC / MS);(ii) has 1H NMR values of d 9.25, 8.36, 8.06, 7.82, 7.71, 7.52, 7.45, 6.82, 6.36, 6.08, 5.42, 5.39, 5.30, 5.14, 4.68, 4.42, 4.31, 4.16, 4.11, 4.07, 3.95 - 3.86, 3.83, 3.72, 3.66, 3.53, 3.48, 3.37, 3.17, 3.06, 2.56, 2.53, 2.45, 2.32, 2.21, 2.02, 1.96, 1.84, 1.72, 1.65, 1.61, 1.51, 1.48 - 1.37, 1.32, 1.12 , 0.94, 0.91, 0.68 and (c) has a retention time (High Pressure Liquid Chromatography) (HPLC) of about 50-55 min. on a C-18 HPLC reverse phase column using a water gradient solvent system: acetonitrile (0-10 min .;30-40% CH3CN water, 10-20 minutes;40-60% underwater CH3CN, 20-60 min;60-80% CH3CN water, 60-65 minutes;(b) The molecular weight of one (I) compound is about 1310-1335, which is determined by LC / MS; and (b) one (I) compound is about 1310-1335; (II) its retention time (HPLC) is about 55-60 minutes, in C-18 HPLC reversed phase column using gradient water solvent system: acrylonitrile (0-10 minutes);30-40% CH3CN water, 10-20 minutes;4-60% underwater CH3CN, 20-60 minutes;60-80% CH3CN water, 60-65 minutes;(c) The molecular weight of a compound (I) is about 540-550, which is determined by LC / MS; (I) in a C-18 HPLC reversed phase column using a solvent system, the retention time (HPLC) is about 50-55 minutes: acrylonitrile (0) or acrylonitrile (0); -10 minutes.35-45% CH3CN water, 10-20 minutes;45-60% underwate
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