A device for analyzing rheotaxis performance of larval fish and a method therefor are provided. The device includes: a bottom plate； a test slot disposed on the bottom plate, wherein the test tank includes a plurality of flow channels in which a plurality of larval fish could swim and a plurality of flow rate controllers； a water storage tank connected with a pump and the plurality of flow rate controllers； a first support portion and a second support portion disposed between the bottom plate and the test tank； and an analysis portion including a photographic component and an analytical component.本發明提供一種用以測試魚苗逆游能力的裝置與方法。所述裝置包含底板；設置於底板上的測試槽，所述測試槽包含用於使複數條魚苗於其內游泳的複數個流道以及複數個流速控制器；與幫浦及複數個流速控制器連接的儲水槽；設置於底板與測試槽之間的第一支撐部以及第二支撐部；以及包含攝影元件以及分析元件的分析部。
