Arizona Board Of Regents On Behalf Of Northern Arizona University;The Translational Genomics Research Institute;THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
发明人:
申请号：
EP14840432.0
公开号：
EP3038649A4
申请日:
2014.08.26
申请国别(地区):
EP
年份:
2017
代理人:
摘要：
The present invention provides a method of detecting a heteroresistant population of a pathogen in a sample, the method comprising: a) providing a sample comprising a population of a pathogen； b) extracting nucleic acids from the sample； c) amplifying a target locus of the genome of the pathogen in the extracted nucleic acids, wherein the target locus comprises at least one minor variant associated with drug resistance in the pathogen； d) consecutively sequencing both overlapping nucleic acid strands from a single DNA molecule amplified from the target locus on a Next Generation Sequencing (NGS) platform； e) applying an alignment algorithm to sequencing data from the overlapping nucleic acid strands； and f) performing an analysis of the aligned sequencing data to detect the at least one minor variant and heteroresistant population of the pathogen.
