A method and a device for autografting of fat microparticles containing stem cells of various types, injected into scars, or stretch marks, and/or other cutaneous injuries, at epidermis-dermis level is presented. Fat is taken from the patient in the periumbilical area, where large quantities of mesenchymal stem cells exist. After reduction in size by means of a thin grid emulsifier filter the fat microparticles are loaded into a tank of a multi-needle gun, such as used for tattoos. The multi-needle tattoo gun injects the fractionated fat microparticles into the epidermis-dermis of scars, and/or stretch marks and/or other skin defects or even internal injuries. The injected fat particles trigger tissue regeneration, deleting scars, stretch marks, wrinkles and/or other defects or skin damages in 3-4 weeks. This method for regeneration of tissues can be extended to all surgeries, including vital organs of the human body； always by means of tattooing the target organ with autologous fat microparticles containing stem cells, wherein access is through open, endoscopic, and/or laparoscopic surgery.
