An object is to provide a method of manufacturing a transdermal absorption sheet and a transdermal absorption sheet that can suppress generation of air bubbles. In the method of manufacturing the transdermal absorption sheet that includes a drug solution filling step, a drug solution drying step, a base solution filling step, a base solution drying step, and a peeling-off step in that order, each step of at least from the drug solution filling step to the base solution drying step is performed in an environment with a temperature of 1° C. to 10° C.
