PROBLEM TO BE SOLVED: To provide isolated DNA encoding enzyme I-SpomI, its recognition and cutting site. SOLUTION: The DNA sequences encoding restriction enzyme I-SpomI having specificity can be incorporated in cloning and expression vectors, transformed cell lines and transgenic animals, from fission yeast (Schizosaccharomyces pombe). The vectors are useful in gene mapping and site-directed insertion of genes. COPYRIGHT: (C)2009,JPO&INPIT