A manufacturing method of plant culture medium includes following steps: (a) isolating a first isolate from a first substance wherein the first isolate contains macro elements (b) isolating a second isolate from a second substance wherein the second isolate contains trace elements (c) mixing the first and second isolates according to a weight ratio into a homogeneous mixture (d) exposing the mixture to heat so as to dissolve the first and second isolates and obtain a solute (e) placing the solute into a mold (f) cooling the solute in the mold into a plant culture medium. The resulting medium, when used in place of water-containing soil, helps to prevents external influences and improve the yield and quality of the plant cultivated therein.本發明係提供一種可用於栽種植物之載體之製作方法,其包括下列步驟:(a)由一第一物質分離出一包含有大量元素之第一分離物;(b)由一第二物質分離出一包含有微量元素之第二分離物;(c)將第一分離物及第二分離物,依重量比均勻調配形成一混合物;(d)將混合物進行熱處理,以溶解第一分離物及第二分離物獲得一溶解物;(e)將溶解物倒入一模具;(f)再對溶解物進行降溫處理形成一載體。藉此,利用載體取代土壤及水源,避免外界環境之影響,可進而提高農作植物之產量及品質。10‧‧‧第一物質11‧‧‧第一分離物20‧‧‧第二物質21‧‧‧第二分離物30‧‧‧混合物40‧‧‧溶解物50‧‧‧模具60‧‧‧載體