The present invention relates to a pharmaceutical composition for promoting arteriogenesis, and preparation method and applications for the same, wherein said pharmaceutical composition comprises an effective amount of a drug, and a peptide hydrogel, and it forms a microenvironment for autologous cell recruitment and tissue regeneration.本發明係關於一種可促進動脈新生之醫藥組成物、及其製備方法與應用,其中前述醫藥組成物包含一有效量之藥物以及一胜肽水膠,且係用於形成一可吸引自體細胞進駐而進行組織再生的微環境。