PROBLEM TO BE SOLVED: To sterilize properly.SOLUTION: When performing sterilization by using a sterilization member (1) including a treatment surface (1A) provided on the surface with a plurality of projection parts each having a thinner tip than a diameter of bacterium which is a sterilization object, a sterilization member (1) with projection parts each having a height of a half or more of the diameter of the bacterium which is the sterilization object is used, and a fluid (5) containing the bacterium which is the sterilization object is allowed to collide with the treatment surface (1A) of the sterilization member (1), to thereby kill the bacterium which is the sterilization object.SELECTED DRAWING: Figure 5【課題】好適に殺菌を行う。【解決手段】殺菌対象の菌の径よりも細い先端を有する突起部が表面に複数設けられた処理面（１Ａ）を備える殺菌部材（１）を用いて殺菌を行うのに、殺菌部材（１）として、突起部が殺菌対象の菌の径の１／２以上の高さを有するものを用い、殺菌対象の菌を含有する流体（５）を殺菌部材（１）の処理面（１Ａ）に対して衝突させて殺菌対象の菌を殺菌する。【選択図】図５
