The present invention utilizes the two-stage floating surface liquid culture with perfluorocarbon oxygen carrier technique (TSF-PT) to significantly improve the content of the bioactive ingredients composition of a new isolate H101 that isolated from wild medicinal herbs DongChongXiaCao (Ophiocordyceps sinensis anamorph Hirsutella sinensis) with benefit both for lung as well as to disclose its production method.本發明利用二階式浮面液態培養-全氟化碳氧載體複合技術(TSF-PT)可顯著提高具有益肺之冬蟲夏草菌(Ophiocordyceps sinensis 無性世代為Hirsutella sinensis)新分離株H101菌絲體生物活性成分組合物含量,並揭示其產製方法。