Methods and test kits are provided for assessing effectiveness of an individual's hand cleansing technique. One method includes donning a glove on one hand and another glove on the other, which then are engaged for a desired period of time. The gloves are removed and the hands cleansed. Thereafter, a third glove is donned on one hand and a fourth glove on the other, which again are engaged. Alternatively or additionally, at least one fingertip on one hand optionally can be swabbed with a swab device to collect a sample from the fingertip, then the hands cleansed. At least one cleansed fingertip can be swabbed with a swab device to collect a sample. The gloves and/or test swab(s) can be sent to a lab and tested for a microbe presence to assesses effectiveness of an individual's hand cleansing technique. Kits can be provided to aid in carrying out said methods.
