A bio-signal analysis method uses a bio-signal analysis apparatus for analyzing a bio-signal based on output light emitted from an object after being output from a light source, and includes a step of providing a classification unit machine-trained based on training data in which measurement values for each wavelength of the output light and concentration for each of a plurality of chromophore materials match each other； a step of inputting the measurement values of the output light emitted from the object measured by the bio-signal analysis apparatus into the classification unit； and a step of outputting chromophore concentration output from the classification unit with respect to the input. At this time, the light source includes a plurality of light emitting elements for emitting output light having different wavelengths.
