BOBROWICZ PIOTR,HAMILTON STEPHEN R.,GERNGROSS TILLMAN U.,WILDT STEFAN,CHOI BYUNG-KWON,NETT JUERGEN HERMANN,DAVIDSON ROBERT C.,Bobrowicz Piotr,Hamilton Stephen R.,Gerngross Tillman U.,Wildt Stefan,Choi Byung-Kwon,Nett Ju
申请号：
SI200431362
公开号：
SI1597381T2
申请日:
2004.02.20
申请国别(地区):
SI
年份:
2013
代理人:
摘要：
The present invention relates to eukaryotic host cells, especially lower eukaryotic host cells, having modified oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar and sugar nucleotide transporters to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified lipid-linked oligosaccharides are created or selected. N-glycans made in the engineered host cells exhibit GnTIII, GnTIV, GnTV, GnT VI or GnTIX activity, which produce bisected and/or multiantennary N-glycan structures and may be modified further by heterologous expression of one or more enzymes, e.g., glycosyltransferases, sugar, sugar nucleotide transporters, to yield human-like glycoproteins. For the production of therapeutic proteins, this method may be adapted to engineer cell lines in which any desired glycosylation structure may be obtained.
