The invention relates to a recombinant virus, which is engineered to contain ahuman tumor suppressor gene, and its application. This recombinantconstructed by DNA cloning technology is combined with virus vector andhuman tumor suppressor gene expression cassette, which can amplify andpropagate in specific genetic-engineered cell lines, and also can expresstumorsuppressor protein in eukaryote cells. In this invention, the recombinantadenovirus-p53 DNA is constructed with adenoviral vector and human p53 geneby homologous recombination in prokaryotic cells (E. coli). The human p53 geneexpression cassette is the specific sequence, which is composed ofpromoter-p53 cDNA-poly (A). The recombinant p53 adenovirus of this inventioncan be prepared as clinical gene therapy products, which apply in treating andpreventing human malignant tumors.
