JOSÉ LUIS MARTÍN PÉREZ SANTOS,JESÚS MUÑOZ ROJAS,YAIR OLOVALDO SANTIAGO SAENZ,ANA LAURA HERNÁNDEZ TENORIO,YOLANDA ELIZABETH MORALES GARCÍA,VERÓNICA QUINTERO HERNÁNDEZ,ANTONIO BAEZ ROGELIO
申请号：
MX2015014804
公开号：
MX2015014804A
申请日:
2015.10.21
申请国别(地区):
MX
年份:
2017
代理人:
摘要：
The present disclosure is related to a method for obtaining healthy potato seedlings inoculated with beneficial bacteria, through an economic, easy to implement and efficient process. Contemplating the sprout induction in potato tubers, under complete darkness conditions, a relative humidity (RH) of 70% and 25°C then the stains are removed and inoculated with a mixture of three beneficial bacteria producing inhibitory substances (EMMIM-5: comprised of Gluconacetobacter diazotrophicus PA15, Pseudomonas putida KT2440 and Sphingomonas sp. OF178). The inoculated sprouts are placed in bottles with sterile vermiculite, where they grow under plant chamber conditions with a 16/8 light/dark photoperiod, at 28°C/25°C, at 80% RH. The seedling growth of inoculated sprouts is healthy and good-looking, in contrast, the seedlings of uninoculated sprouts are easily contaminated with fungi. The generated seedlings can be transplanted to larger pots under greenhouse conditions or be cultivated in the field. The method of this invention has been referred to as EIBP-EMMIM-5, that means: Extraction and inoculation of potato sprouts with the EMMIM-5 bacteria mixture.La presente invención describe como obtener plántulas de papa sanas e inoculadas con bacterias benéficas, mediante un proceso económico, fácil de llevar a cabo y eficiente. El proceso contempla la inducción de brotes en tubérculos de papa, bajo condiciones de obscuridad, 70% de humedad relativa (HR) y temperatura de 25 oC, después se extraen los brotes y son inoculados con una mezcla de tres bacterias benéficas productoras de sustancias inhibitorias (EMMIM-5: conformado por Gluconacetobacter diazotrophicus PAl5, Pseudomonas putida KT2440 y Sphingomonas sp. OF178). Los brotes inoculados se colocan en frascos con vermiculita estéril, donde crecen bajo condiciones de cámara de plantas con un fotoperiodo luz/obscuridad 16/8, a 28 oC/25 oC, a 80% HR. El crecimiento de plántulas de brotes inoculados es sano y de buena apariencia en
