Former culture method of the human embryo characteristic trunk cell needed the nutrient medium which discloses to the feeder tissue bud cell or the feeder tissue bud cell in order to maintain the aforementioned trunk cell in not yet differentiation state. When the antagonist of the bone formation protein, with tissue bud cell growth factor is added to the nutrient medium which cultures the trunk cell, even if the feeder cell or the conditional nutrient medium did not exist and as for the trunk cell now it made also the te, clear that it continues to be not yet differentiation unlimited.ヒト胚性幹細胞のこれまでの培養方法は、前記幹細胞を未分化状態で維持するためにフィーダー線維芽細胞又はフィーダー線維芽細胞に暴露した培地を必要とした。骨形成タンパク質のアンタゴニストを、幹細胞を培養する培地に線維芽細胞増殖因子とともに添加すると、たとえフィーダー細胞又は条件培地が存在しなくても、幹細胞は無限に未分化のままであることが今や明らかにされた。
