Gregory J. Ewing,Khairuzzaman Bashar Mullah,Ronald Graham
申请号：
US13629021
公开号：
US08637658B2
申请日:
2012.09.27
申请国别(地区):
US
年份:
2014
代理人:
摘要：
Bis-diazo, triaryl and aryldiazo-N-arylphenazonium quencher moieties, substituted with electron-withdrawing and electron-donating substituents which induce polarity in the delocalized aryl/diazo ring systems, are useful as labels when attached to biomolecules such as polynucleotides, nucleosides, nucleotides and polypeptides. The quencher moieties are non-fluorescent and accept energy transfer from fluorescent reporter labels by any energy-transfer mechanism, such as FRET.Fluorescence quencher compositions are useful in preparing quencher labeled biomolecules for various molecular biology assays based on fluorescence detection.
