NZ 600278 Disclosed is an isolated von Willebrand factor (vWF) polypeptide comprising a Factor VIII (FVIII) binding domain, with the proviso that the polypeptide lacks one of vWF domain A1, A2, A3, D4, B1 , B2, B3, C1, C2, CK, or a combination thereof, wherein the polypeptide further comprises an immunoglobulin Fc amino acid sequence comprising at least a hinge region, wherein the polypeptide exhibits binding to the FVIII, wherein the Factor VIII (FVIII) binding domain comprises the amino acid sequence as set forth in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:33, SEQ ID NO:34, or SEQ ID NO:35, wherein the sequences are disclosed in the specification. Also disclosed is a method for preparing the isolated von Willebrand factor (vWF) polypeptide and its use in the manufacture of a medicament for enhancing a plasma pharmacokinetic property of a FVIII.
